Tail Rotor Shaft (TRS) is a critical part in Helicopter Transmission System, and its loads are very complicated. This tail rotor shaft is made up of Hub and shaft, which incorporate structure design method used. During the flight, tail rotor shaft should suffer a type of large centrifugal load, due to three high-speed circumrotating. And the life of tail rotor shaft should be affected by the centrifugal load. This article discusses a method that considering the complicated structure of a tail rotor shaft, a set of special centrifugal load applying device and multi-load coordination fatigue test technology are used and carried out the centrifugal load applying triumphantly.