The vibration loads will be produced between wheel and rail on the running of the high-speed Train. The vibration energy will be transferred to the ground formation through the rail, guiding bed and tunnel lining structure, thereby causing vibration between the formation and surface and environmental interference effect problems. Thus the research of related issues caused by the high-speed train vibration has the vital great significance. The train design speed of the Shiziyang shield tunnel for Guangzhou-Shenzhen-Hong Kong passenger dedicated line is up to 350km/h. In this paper the research object is located in the segment of the homogeneous formation of the Shiziyang shield tunnel. The analysis of this paper includes the dynamic response rules of the shield tunnel and formation under the single high-speed train loads and the law of the pore water pressure accumulation and dispersion under train cyclic loading.