Nowadays, delays problem are important issue in preparing schedule for public transportation. It is related to behaviour of passengers, behaviour of transportation means and interaction with traffic. Behaviour of passengers are including: demand patterns of passengers, fare payment and waiting time in station and in-vehicle. Behaviour of transportation means included fleet size and frequency of operation. The most interaction between public transportation and others traffic is in traffic congestion. In this paper, we studies different behaviour of passengers duration operation time in two points: in-station and in-vehicle. They are including: arrival rate of passengers, kinds of fare payment and waiting time. For illustrate behaviour of passengers on delays problem we survey they affects in bus operation. Hence, a case study based on an actual public bus operation in Tehran, Iran is used to demonstrate it.