In order to improve the reliability of urban road network operation evaluation, the road network regional Partition methods were launched in this paper. The geographic grid was introduced first, and a 4-level road network model was defined. Then, the spatial analysis based urban road network division method was proposed by analyzing the characteristics of road network operation. This method can reflect the influence between adjacent regional units, and improve the reliability of urban road network division. Finally, this research took a certain area in Beijing as a case study, and divided the road network as several regional units. Macroscopic evaluation result shows that it is effective for scientifically describing the road network operation status.