Vanadium oxide nanotubes (VOX-NT) have been synthesized by using amine as structure-directing template and V2O5 as precursor under hydrothermal conditions (160°C, 7 days). The XRD, FTIR and TEM have been performed to optimize the synthesis conditions such as the amine species and the molar ratio between V2O5 and amine. The results of TEM show that the same molar ratio of V2O5 and butylamine (1.0:1.0) produce a mass of nanotubes which have intact figuration. The diameters are about 80nm for most of the nanotubes prepared by V2O5 and octadecylamine. While the obtained VOX-NT prepared by V2O5 and n-butylamine were found having diameters from 28.51nm to 97.80nm and the distance between the vanadium oxide layers is 1.84 nm comfirmed by XRD. FTIR also display that some changes of the structure have been occurred during the formation of the nanotubes compared with the staring materials.