wood is the most abundant renewable resource and environmentally friendly energy source on the earth, it not only provides industrial raw materials for economic and social sustainable development, meanwhile, the biological process of wood formation which is mainly to sink the excessive carbon dioxide in the atmosphere can also play an active role in reducing “greenhouse effect”, so it is the contributor of green environment and human heath. Therefore, it is of great importance to explore the biosynthesis process and the wood formation mechanism of woods cellulose. This study adopted RT-PCR to clone gene fragments from the total RNA of populus ussuriensis secondary xylem, through sequence analysis, we found that its size was 487bp, which was named as PusC1，by means of blast comparative analysis, we found that the gene sequence similarity of this fragment and Populus trichocarpa cellulose synthase (XM 002305024.1) reached 94%, and its gene sequence similarity with Populus tremuloides PtrCesA1 and Populus tremula × Populus tremuloides xylem specificity cellulose synthase genes (AY573574.1) sequence could also reach 92%. Therefore, it is inferred to be populus ussuriensis xylem specificity cellulose synthase gene fragment. Through sequence similarity, we can also infer that populus ussuriensis has a close genetic relationship with Populus trichocarpa and belongs to cathay poplar species; while Populus tremuloides belongs to white poplar species and has a close genetic relationship with populus ussuriensis.