The paper purposes is explore the forefront information of a large-scale protein production from alfalfa cells. With five varieties alfalfa, seleted the highest cell medium from callus . and detected the biomass and protein content in cell grows from different nitrogen source and sucrose concentration. Rusult is the highest protein in 12th -18th days of grows. There are different protein content in different nitrogen source (from Fig.2) and in different sucrose concentration range medium(from Fig.3).Conclusion is obtained the protein from alfalfal cells that is possibally.