Straw has a complex structure composed mainly by cellulose, hemicellulose and lignin, resulting in the difficulty to use it as the substrate for hydrogen production directly. Hydrogen production from saccharification plant straw was performed in this study. The effect of experimental time and temperature on saccharification efficiency of the straw was investigated in this work. The results show that efficiency of saccharification by hydrochloric acid on plant straw is better than that by acetate due to the different extent of acid ionization. The maximum sugar content of 36.8 Brix and 35.4 Brix was acquired at the experimental time of 1h and temperature of 20°C with the corresponding hydrogen yield of 0.02mlH2/L saccharification liquid and 0.0182mlH2/L saccharification liquid by bacteria SUES-1, respectively.