In this paper, the hydrophobic nano-calcium carbonate (CaCO3) was prepared by organic matter (the bolaform surfactant Disodium Sulfodehydroabietate as template) modifying surface structure of calcium carbonate (made by anhydrous sodium carbonate and anhydrous calcium chloride) at 40°C for 2h. The prepared hydrophobic nano-CaCO3 was characterized by means of Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission electron microscope (TEM) and. The nano-CaCO3 with mean particle size of 64.4 nm was obtained in the range of 0.5~1.5µm by calculation. The contact angle of nano-CaCO3 with liquid solid was 117.49°. The hydrophobic property become stronger.