Paper Title:
Purification and Characterization of Laccase from Curvularia trifol
  Abstract

Electrophoretic pure lacquer enzymes were obtained from the thick enzyme of Gladiolus Curvularia trifol with grading precipitation and DEAE2-Cellulose ion exchange chromatography. The overall recycling rate of enzyme activity was 14.97% and the purification reached 2.96 times. The relative molecular mass of enzyme was 92.3Kda. The optimal temperature and pH were 40°C and 3.5, respectively. The Km of ABTS catalyzed by laccase was 1.11× 10- 5mol L-1. Alizarin red and Congo red could be degradated by purified laccase effectively without the participation of small molecule amboceptor. Alizarin red could be degradated by 80% with its being affected by 1000U/L of enzyme activity for 70h, which revealed the huge potentiality of Gladiolus Curvularia trifol and laccase in the degradation of dye.

  Info
Periodical
Advanced Materials Research (Volumes 113-116)
Edited by
Zhenyu Du and X.B Sun
Pages
2215-2219
DOI
10.4028/www.scientific.net/AMR.113-116.2215
Citation
J. Zhang, J. Ren, Y. Yu, B. S. Wang, "Purification and Characterization of Laccase from Curvularia trifol", Advanced Materials Research, Vols. 113-116, pp. 2215-2219, 2010
Online since
June 2010
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jie Zhang, Yang Yu, Jing Ren, Bin Song Wang
Abstract:Macroporous exchange resins have been applied as supports for enzyme immobilization. The supporters were activated by cross-linking with...
2115
Authors: Da Jun Ren, Xin Bao, Li Hua Liu, Qin Xu, Shu Qin Zhang, Gao Ming Wu
Chapter 7: Pollution Control Project (Air, Water, Solid), Removal and Treatment Technologies
Abstract:NH4+-N was choiced as an influence factor for analysis the effect on the laccase activity from the white rot fungus...
2274
Authors: Bei Bei Zhang, Yong Jian Shen, Chang Xu, Ying Li, Ming Hu Jiang
Chapter 2: Applied Materials in Industry
Abstract:Dyes are usually difficult to be decolorized due to their complex chemical structures. In this work, laccase was purified from the white rot...
120