The microbial fuel cell (MFC) constructed by a modified Continuous Stirred Tank Reactor (CSTR) which was used as acidification-phase of two-phase anaerobic digestion system. The experiment was operated as batch mode at mesophilic condition (35°C) to evaluate continue voltage output using cattle dung as substrate in hydrolysis-acidification process. The results illustrated that electricity generation increased noticeably to 300mV after 3 days operation, reached 430mV after 20 days and stabilized electricity generation from 420mV to 470mV in the following 70 days. The pH decreased from 7.15 to 6.65 after 15 days operation and maintained stability from 6.4 to 6.8. The main components of VFA in anode chamber were acetic, propionic and butiric acids. The dominating VFA was acetic acid that predominated untile day 50 and the maximum propionic acid concentration was 15% of total VFA.