Bacterial strains with chlorimuron-ethyl degrading ability were isolated for bioremediation of contaminated soil. Six strains were obtained from chlorimuron-ethyl contaminated soil by enrichment cultivation. HPLC analysis indicated that two strains (A4 and A5) demonstrated high degradation efficiency than other strains. More than 61% of chlorimuron-ethyl was degraded by the two strains after 24 h. Based on the results of biochemical tests and 16S rDNA sequence analysis, the strain A4 and A5 were identified as Bacillus licheniformis and B. cereus, respectively. The cultivation conditions of the two strains were optimized to increase the biomass production.