Very high cycle fatigue behavior of high strength steel, were investigated using ultrasonic fatigue testing equipment at 20 kHz up to 109cycles. S-N curves at room temperature with different stress ratio (R=0.01 and R=0.1) was determined. The experimental results show that fatigue strength decrease with increasing number of cycles between 105 and 109. SEM examination of fracture surface reveals that fatigue damage was governed by the formation of cracks, and subsurface crack initiation was in the very long life range. The results shown that the portions of life attributed to subsurface crack initiation between 107 and 109 cycles are 99%.