Sodium alginate (SA) is a polyanionic copolymer consist of mannuronic(M) and guluronic(G) sugar residues, it has been comment used in biomedical applications. In this research, polylactic acid (PLA) and Tencel fiber were fabricated into PLA/Tencel composite nonwoven fabric. Then the gel aqueous Ca-SA was combined with PLA/Tencel composite nonwoven fabric by three-dimensional (3D) coating method to form porosity coating layer. Then LMWCS was sprayed on the whole surface to form antibacterial layer. The physical properties of novel wound dressing were evaluated such as tensile strength, tear strength, water absorption rate, stiffness and SEM. In SEM observation result indicated, the coating layer performs highly porosity structure. Between coating layer and PLA/Tencel composite nonwoven fabric was combined by physical bonding. In this way, PLA/Tencel composite nonwoven fabric can remain some part of air permeability and also enhance the strength of dressing.