In this paper, the poplar LVL was reinforced with monolayer fiberglass mesh. The reinforcing effect of lay angle between the weft of fiberglass mesh and the veneer grain was studied. And three different nondestructive testing (NDT) methods, such as the longitudinal transmission method, longitudinal vibration method and flexural vibration method (out-plane and in-plane), were used to test the dynamic Young’s modulus of the reinforced poplar LVL. The correlativity was investigated between the dynamic Young’s modulus and the static MOE of the reinforced poplar LVL. The reinforcing effect was best when the lay angle of fiberglass mesh was 30°. And the flexural vibration method and longitudinal vibration method had better accuracy to estimate the static MOE.