Weldability analysis, metallographic experiments and mechanical property experiments were carried out on weld joint between B610CF and 16MnR steel using shielded metal arc welding method and mixed active-gas arc welding method. Weldability analysis shows that the weld joint has some tendency to cold crack, and preheat is needed before welding. Metallographic results show that there are ferrite and bainite in weld metal, and in heat-affected zone of B610CF side there are ferrite and bainite, on which there is much dispersed slight Fe3C, and in heat-affected zone of 16MnR side there are ferrite, pearlite. There is no quenching microstructure resulting in crack in weld joint. From mechanical property results, it can be concluded that the weld joints have excellent impact toughness at low temperature and the tensile strength and plasticity of weld joints is matched to the ones of 16MnR steel. So the welding parameters in this paper are appropriate to get qualified weld joints.