Expanded polytetrafluoroethylene (ePTFE) is a bioinert material. To improve the ePTFE film biocompatibility, the cold plasma technology was used with acetic acid as monomer to deposit onto ePTFE film and then (N-isopropylacrylamide) was grafted onto the surface by photo-grafting. The characteristics of the surface were evaluated with X-ray photoelectron spectroscopy (XPS), FTIR and water contact angle. It was found that the contact angle of water on the untreated ePTFE significantly decrease from125° to 72° after ePTFE film being treated with acetic acid plasma deposition treatment. Due to the hydrophilicity of poly(N-isopropylacrylamide), the contact angle of water on the ePTFE-g-NIPAAm approached to 0°.