Engineering Biomaterials Surfaces Using Micropatterning |
| Journal |
Advanced Materials Research (Volumes 15 - 17) |
| Volume |
THERMEC 2006 Supplement |
| Edited by |
T. Chandra, K. Tsuzaki, M. Militzer and C. Ravindran |
| Pages |
77-82 |
| DOI |
10.4028/www.scientific.net/AMR.15-17.77 |
| Online since |
February, 2006 |
| Authors |
Louis Gagne,
Gaetan Laroche
|
| Keywords |
Arterial Tissue Engineering, Bioprosthesis, Cell Adhesion, Cell Proliferation, Cell Spreading, Endothelial Cell, Endothelialization, Micropatterning, Peptide, Surface Conjugation, Surface Modification, Thrombogenicity |
| Abstract |
A new technique for micropatterning surfaces for cell growth support is described
and characterized. This technique allows covering of large three-dimensional surfaces at low
cost with controllable micropatterns. This method takes advantage of the random properties of
aerosols and the principles of liquid atomization. Parameters of interest were the pressure of
atomization air, the flow rate and volume of the atomised liquid, and the distance between the
spray nozzle and the surface of the sample. The experimental setup permitted to obtain mean
diameters of spots between 10 and 20 microns with a maximum surface coverage of 20%. In
an initial step, polytetrafluoroethylene (PTFE) films were treated with ammonia plasma to
insert amino groups on the surface. The ammonia plasma treated films were immersed in a
solution containing sulfosuccinimidyl 4-(N-maleidomethyl)cyclohexane-1-carboxy-late (SSMCC)
to permit the introduction of maleimido groups on the PTFE surface to subsequently
conjugate peptides through a sulfhydryl containing N-terminal cystein residue.
Plasma/S-SMCC pretreated surfaces were then sprayed with peptide sequences CGRGDS and
CWQPPRARI. Our data showed that spots of CGRGDS peptides over a background of
CWQPPRARI peptides were the most effective combination to enhance endothelialization. |
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