This paper investigated the preparation and characterization of the Zn-antibacterial White Carbon Black modified by electrolyte, surfactant, and organic solvent. The sol-gel method was used to prepare the White Carbon Black carrier. Zn2+ was selected to be the antibacterial ion, and Sodium nitrite (electrolyte), Sodium dodecylbenzene sulfonate (Surfactant), Ethanol (organic solvent) was selected to be the modifiers. They were synthesized on the white carbon black carrier. These as-synthesized modified antibacterial materials were characterized by inductively coupled plasma, particle size measurement instrument and enumeration tests (Escherichia coli as experimental bacterium). The result showed that the amount of antibacterial ions and bacteriostasis rate of modified Zn-antibacterial white carbon black are higher than those for the common Zn-antibacterial white carbon black. In addition, the particle sizes of modified samples can be extended down with a narrow size distribution. Other advantage of modified Zn-antibacterial white carbon black is good thermal stability.