Paper Title:
Purification and Characterization of a Natural Lectin from the Seed of Peanut Arachis hypogaea
  Abstract

A natural lectin from the seed of peanut Arachis hypogaea was purified by singlestep affinity chromatography using galactoside-coupled agarose. This lectin was named PN-L and its inactive form had a molecular mass estimate of 29 kDa. The lectin PN-L was detected for agglutinating activity, glycoinhibiting action and thermostability. The influence of pH on those activities was also tested. The results showed that PN-L could not agglutinate three kinds of human erythrocytes. But it showed a strong affinity for human A/B/O erythrocytes (RBC) by neuraminidase treated. Agglutinating activity of PN-L to neuraminidase treated human O erythrocytes was inhibited by lactose , raffinose, melibiose and D-galactose. The agglutinating activity of peanut seed lectin was inhibited at temperatures greater than 55 and at a pH less than 5 or greater than 11.

  Info
Periodical
Advanced Materials Research (Volumes 152-153)
Edited by
Zhengyi Jiang, Jingtao Han and Xianghua Liu
Pages
1499-1504
DOI
10.4028/www.scientific.net/AMR.152-153.1499
Citation
J. Sun, Q. L. Yang, J. Bi, C. S. Zhang, L. N. Yu, F. Zhu, "Purification and Characterization of a Natural Lectin from the Seed of Peanut Arachis hypogaea", Advanced Materials Research, Vols. 152-153, pp. 1499-1504, 2011
Online since
October 2010
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