This study tested four types of fireproof and stuffing materials, including firestop sheets, silicone fire barrier sealant, mineral wool batt, and double agent fire barrier foam, on through-floor pipes and walls. Based on the specifications of the American society for testing and materials (ASTM) E84, a 10 min substrate surface burning test was conducted to test fireproof and stuffing materials. The results indicated that the level of fireproof material substrates reached the level of class A in ASTM E84. Combination methods A and B were tested by 180 min heating for specimens, according to the ASTM E814 standard temperature-time (T-t) heating curve, followed by the water spraying test. The results showed that methods A and B have 180 min of fire prevention effects (F rating), and method B can achieve 180 min of heat prevention effects (T rating).