Paper Title:
A Novel Scaffold from Recombinant Spider Silk Protein in Tissue Engineering
  Abstract

As a new biomaterial, recombinant spider silk protein has attracted much attention in tissue engineering. The pNSR-16/ BL21(DE3)pLysS strains fermented and produced the recombinant spider silk protein, which was then cast into scaffolds. NIH-3T3 cells were cultivated with extractions of the scaffolds in vitro. The cytotoxicity of scaffolds was analyzed with a MTT assay. The performances of cells adhesion, growth and expression on the scaffolds were observed with SEM, HE staining and immunohistochemistry. Compared with the control, the extract fluid of materials culturing the NIH-3T3 cells was not apparently different. NIH-3T3 cells could adhere and grow on the scaffolds and secret FGF-2. The pNSR-16 recombinant spider silk protein scaffolds has satisfactory cytocompatibility and the scaffolds are ideal scaffold material for tissue engineering.

  Info
Periodical
Advanced Materials Research (Volumes 152-153)
Edited by
Zhengyi Jiang, Jingtao Han and Xianghua Liu
Pages
1734-1744
DOI
10.4028/www.scientific.net/AMR.152-153.1734
Citation
H. X. Wang, Z. X. Xue, M. H. Wei, D. L. Chen, M. Li, "A Novel Scaffold from Recombinant Spider Silk Protein in Tissue Engineering", Advanced Materials Research, Vols. 152-153, pp. 1734-1744, 2011
Online since
October 2010
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Price
$32.00
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