The relationships between the PANI content and the surface resistance of PANI composited conductive fabric were established by the design of quadratic general rotation combination. In the experiments, the five major process parameters such as concentration of hydrochloric acid, ammonium persulfate, oxidation time, the power and the time of ultrasonic immersion, were taken into account as the independent variables, then the regression equations were established. The PANI content and the surface resistance of conductive fabric in response to values, which is validated with experiment, the result proved that a high degree of the curves fitting mathematical model for which is found. These equations were employed to study the relation between the five process parameters and the PANI content and the surface resistance of the conductive fabric. In order to prepare a good conductivity of conductive fabric with minimum surface resistance, the five main process parameters were optimized, and obtained the optimum conditions with concentration HCI at 1.3 mol • L-1, concentration of APS at 0.02 g • mL-1, oxidation reaction time at 90min, ultrasonic immersion power at 0.5 W • cm-2 and ultrasonic immersion time at 100 min.