Strontium hydroxyapatite powders was prepared by the hydrothermal method using Sr(NO3)2 and (NH4)2HPO4 as reagents. Fourier transform infrared spectroscopy, X-ray diffraction, Transmission electron microscope, Energy dispersive X-ray, and Thermogravimetric-differential thermal analysis were employed to investigate the crystalline phase, chemical composition, morphology, and thermal stability of the Strontium hydroxyapatite. And the cytotoxicity of Strontium hydroxyapatite was analyzed through MTT assay. Results showed that Strontium hydroxyapatite prepared by hydrothermal Method has excellent crystal structure, good dispersion, high purity, and rod-like morphology with dimensions 200-500 nm in length and 20 nm in diameter. Meanwhile, the apatite has poor thermal stability. However, the apatite is cytocompatible and may have better biocompatibility, which can serve as strontium source incorporation into calcium phosphate cement and for bone repair.