Paper Title:
A Novel Amperometric Immunosensor Based on Thionine/DNA Self-Assembled Multilayers on Carbon Nanotubes Modified Glass Carbon Electrode
  Abstract

A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using multi-wall carbon nanotubes modified glass carbon electrode.thionine as the mediator,were immobilized by DNA throngh the layer-by-layer method. Thionine was first fabricated on carbon nanotubes modified glass carbon throngh 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).then a negatively charged DNA film was absorbed on the positively charged thionine,and the DNA was uesd as cross-linker to immobiled amount thionine.nano-Au-was used to immobilized anti-AFP.The electrochemical sensor have a relatively low detection limit of 0.02ng/ml.

  Info
Periodical
Advanced Materials Research (Volumes 160-162)
Edited by
Guojun Zhang and Jessica Xu
Pages
1170-1175
DOI
10.4028/www.scientific.net/AMR.160-162.1170
Citation
L. Y. Jia, N. Gan, L. Zheng, Q. Wang, "A Novel Amperometric Immunosensor Based on Thionine/DNA Self-Assembled Multilayers on Carbon Nanotubes Modified Glass Carbon Electrode", Advanced Materials Research, Vols. 160-162, pp. 1170-1175, 2011
Online since
November 2010
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