Most soil constitutive models were developed based on the traditional triaxial tests with isotropic assumption, in which the load is applied as the major principal stress direction and the other two principal stresses are symmetric. When such isotropic models are applied to practical analysis, stress induced anisotropy under complex stress state and the middle principal stress effects are often neglected, thus there are many disagreements between the calculated results and the infield testing data. To simulate the practical loading process, true triaxial tests were carried out on geomaterial under three-dimensional stress state. It was found that the stress induced anisotropy effects are remarkable and the middle principal stress effects are obvious because of the initial three-dimensional stress state. Such kind of stress-induced anisotropy could have important impact on the numerical analysis results and should be taken into consideration when developing the constitutive model.