The cyclohexanone production wastewater includes hazardous material such as cyclohexanone and cyclohexane which can harm the vessel of human body resulting in coagulation necrosis of viscera and brain. Our experiment choosed the cyclohexanone production wastewater to be investigated, and used the self-made anaerobic upflow bed filter (UBF) to deal with it. We try to find the optimal operational parameters which can make the degradation of hazardous materials maximizing. The COD removal efficiency was decreasing with the COD of influent’s increasing in starting stage, the shock load made the microbe in UBF can’t adapt the high VLR temporarily. In the running stage, the anaerobic sludge in UBF was incompact and the settleability of sludge was not very well. As the UBF running, the granular sludge shaped up greatly, the COD removal efficiency kept on 80%. The result showed that the UBF reactor was valid for hazardous material in cyclohexanone production wastewater.