Microstructure of A357 alloy modified by Sr has been investigated by the Electron Back Scattering Diffraction (EBSD) mapping technique using a Field Emission Gun Scanning Electron Microscopy (FEG-SEM). An appropriate sample preparation technique by ion milling was applied to obtain a sufficiently smooth surface for EBSD mapping. Results show that the eutectic morphology in microstructure of A357 alloy modified by Sr was changed to fine fibrous, and the grain size was refined. By comparing the orientation of the aluminum in the eutectic to that of the primary aluminum dendrites, the nucleation and growth mechanism of the eutectic solidification in A357 cast alloy was determined. The eutectic Si phase of the modified sample nucleates on the heterogeneous nuclei located in the region between primary α-Al dendrites and grows up, while the eutectic Si phase of the sample without modification nucleates on the primary α-Al dendrites and grows up.