This template Based on cracks observation and finite element analysis of real engineering projects as well as bridge load test after reinforcement, causes and types of cracks in prestressed concrete box girder bridges and treating measurements are systematically studied. The results obtained from the calculation are presented to demonstrate the effect of sensitive factors, such as arrangement of longitudinal prestressed tendons, the magnitude of vertical prestressed force, temperature gradient, etc. The results show that the arrangement of longitudinal prestressed tendons and the magnitude of vertical prestressed force take key roles in cracks control of box girder webs. Lots of treating measurements are presented in accordance with different types of cracks, some of them are applied to a reinforcement engineering of a long span pretressed concrete continuous box girder bridge with cracks. Load test after reinforcement of the bridge demonstrates the reasonability of the treating measurements. Several design recommendations and construction measures about reinforcements and some sensitive factors mentioned above are proposed to control cracks.