Mechanical properties of fiber reinforced concrete with polypropylene fiber, alkali-resistant glass fiber and basalt fiber separately were studied in this paper. The internal structure of fiber reinforced concrete was researched by testing chloride ion diffusion coefficient and scanning electron microscope (SEM) analysis. The results show that adding a certain amount of three fibers separately into concrete have all increased splitting strength. Compared with referenced concrete, compressive strength of alkali-resistant glass fiber reinforced concrete and basalt fiber reinforced concrete are both improved. According to analysis, the effect of srengthening and toughening for basalt fiber is particularly significant. The order of chloride ion diffusion coefficient from lower to higher is alkali-resistant glass fiber reinforced concrete, referenced concrete, basalt fiber reinforced concrete, polypropylene fiber reinforced concrete. This result indicates that alkali-resistant glass fiber bonds cement paste best and makes internal structure densest by SEM analysis.