Under the promoting of digital information process and computer controlling system, modern printing industry comes to a digital workflow era. Digital workflow not only changed conventional prepress and printing process but also changed the organization and transmission of conventional printing information, which brings in both opportunities and challenges to printing industries. Based on Prinergy digital workflow, the control method of prepress data was studied. According to the requirement of printing production, through theoretical analysis and experiments, with analysis of key nodes of data transferring and controlling, and parameter settings and optimization, a set of data flow control template was established, which ensures smooth and accurate transmission of printing data stream in prepress workflow. What has been achieved in this paper has theoretical and practical significance in using digital workflow to improve the printing quality.