Paper Title:
Preparation and Characterization of Silk Fibroin Microspheres
  Abstract

An improved method of water-in-oil-in-water (w1/o/w2) multi-emulsion was developed to prepare silk fibroin (SF) microspheres that had improved drug-loading amount and better drug-loading efficiency. SF and dexamethasone sodium phosphate (DSP) were used as one water phase(w1), liquid paraffin was used as oil phase and isopropanol was used as the other water phase(w2). In the preparation process, isopropanol was also used to induce the crystallization of SF. The SF microsphere was mainly composed of silk II proteins. The drug-loading amount of SF microsphere ranged from 28.45 µg/mg to 79.21 µg/mg, and the drug-loading efficiency varied from 30.80% to 87.16%. The spherical morphology of the microsphere was observed by SEM. The particle diameter was measured by the Laser particle sizer and their average sizes varied from 7.41 µm to 66.10 µm. The drug releasing profile of the microspheres was measured in vitro. The burst release was significant when isopropanol-to-SF ratio was lower than 3:1,although the drug-loading amount and the drug-loading efficiency was high; It showed a higher drug-loading amount and efficiency, as well as an obvious continuous release effect when the ratio was higher than 4:1; It also showed a certain continuous release effect when the ratio was 4:1.

  Info
Periodical
Advanced Materials Research (Volumes 175-176)
Main Theme
Edited by
Lun Bai and Guo-Qiang Chen
Pages
110-115
DOI
10.4028/www.scientific.net/AMR.175-176.110
Citation
R. J. Xie, H. Y. Wu, M. N. Zhu, Y. Y. Huang, "Preparation and Characterization of Silk Fibroin Microspheres", Advanced Materials Research, Vols. 175-176, pp. 110-115, 2011
Online since
January 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Kong Jun Zhu, Jin Hao Qiu, Koji Kajiyoshi, Kazumichi Yanagisawa
Abstract:Hydrothermal method was used to synthesize the Na(TaxNb1-x)O3 (x = 0.1-0.5) powders with the addition of isopropanol. The phases of the...
1899
Authors: Jun Wu Zhu, Tian Jian Cheng, Dong Ping Sun, Qiao Feng Han, Xin Wang
Abstract:A facile method capable of preparing CuO nanocrystals has been developed in a water-isopropanol system. The different shapes of CuO...
1011
Authors: Jing Long Bu, Heng Yong Wei, Jun Hong Zhao, Rui Sheng Wang, Zhi Fa Wang, Rong Lin Wang
Micro/Nano Materials
Abstract:Magnesium aluminate (MgAl2O4) spinel nanoparticles were prepared by non-hydrolytic sol-gel method using anhydrous...
446
Authors: Tao Liu, Feng Zhang, Bao Qi Zuo
Chapter 4: Micro/Nano Materials
Abstract:In this paper, we employed Methanol, 75% Ethanol, 100%Ethanol, and Isopropanol to treat electrospun SF nanofiber mats, and studied the...
607
Authors: Wen Juan Gu, Xiao Hui Zhang, Jie Li
Chapter 2: Materials Science and Engineering
Abstract:Highly uniform polyvinylacetate particles of submicron size were prepared by dispersion polymerization in mixed media of isopropanol and...
1134