Paper Title:
Studies on Recombinant Human Bone Morphogenetic Protein 2 Loaded Nano-Hydroxyapatite/Silk Fibroin Scaffolds
  Abstract

Bone Morphogenetic Protein 2 (BMP-2) is a member of the transforming growth factor superfamily. It plays an important role in stimulating osteoblast differentiation and bone formation, and has been widely utilized in clinical bone repairing by implantation. In this study, the nano-hydroxyapatite (nHA)/silk fibroin (SF) porous scaffolds were fabricated for the sustained delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2), and then used to address the hypothesis that rhBMP-2 delivered from the scaffolds could enhance the bone formation in vitro. We optimized an effective method using a prokaryotic expression system to produce rhBMP-2. The rhBMP-2 was expressed, purified and renatured in vitro. And then the rhBMP-2 was loaded onto the nHA/SF scaffolds. The bioactivities of rhBMP-2-loaded nHA/SF scaffolds were assessed in vitro. The results showed that the rhBMP-2 promoted the osteoblasts adhesion and proliferation on the nHA/SF scaffolds. Also, the rhBMP-2 released from the nHA/SF scaffold stimulated a significant increase in alkaline phosphatase (ALP) activity of osteoblasts in vitro. These results demonstrated that the rhBMP-2-loaded nHA/SF scaffolds could promote the bone regeneration and showed potential applications in the bone tissue engineering.

  Info
Periodical
Advanced Materials Research (Volumes 175-176)
Main Theme
Edited by
Lun Bai and Guo-Qiang Chen
Pages
253-257
DOI
10.4028/www.scientific.net/AMR.175-176.253
Citation
Y. H. Zhang, L. J. Zhu, J. M. Yao, "Studies on Recombinant Human Bone Morphogenetic Protein 2 Loaded Nano-Hydroxyapatite/Silk Fibroin Scaffolds", Advanced Materials Research, Vols. 175-176, pp. 253-257, 2011
Online since
January 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Sun Jung Yoon, Ki Suk Park, Bang Sil Choi, Gil Son Khang, Moon Suk Kim, John M. Rhee, Hai Bang Lee
Abstract:This study was designed to investigate the influence of demineralized bone particles (DBP)/PLGA hybrid scaffold on angiogenesis and...
161
Authors: Tatsuhide Hayashi, Kentaro Yoshihara, Mayu Kawase, Akimichi Mieki, Hiroyasu Kataoka, Soichiro Hamajima, Tatsushi Kawai
IV. Characterization & Testing
Abstract:The aim of this study is to induce bone from immature muscular tissue in vitro using recombinant human BMP (rhBMP)-2 and expanded...
103
Authors: Qing Li, Gang Zhou, Xin Yu, Tong Wang, Zhi Hui Tang
Chapter 2: Bio and Medical Materials and Technologies
Abstract:The bone substation grafts, such as hydroxyapatite, TCP, have been used in the clinical. But it is found that they are not capable of...
187