The fabrics made of different color values of Lightness (L), Chromaticity (C) and hue angle (h) of warp and weft silks were designed to testify color mixing. According to Glassman’s two-color adding and mixing principle, theoretical values of L0, C0 and h0 for all yarns were calculated. After all yarns were weaved, L1, C1 and h1’s value of each fabric were measured by ultrascan PRO of Optoelectronic integration color measurement instrument. The differences between measured and theoretical values expressed by ΔL, ΔC and Δh were calculated. ΔL, ΔC and Δh characteristics were analyzed and the relationship between ΔL, ΔC and Δh and the colors of warp and weft were discussed. The effects of the relative portion of warp and weft silks, the collection and distribution of interweave points, the uniformity of organized points’ distribution on color mixing based on the measured and theoretical values of fabric were further investigated.