Paper Title:
Standardization of Reference Genes in Silkworm, Bombyx Mori
  Abstract

The Bombyx mori serves as model organism among the Lepidoptera insects. In the post-genomic era, in order to study gene function, the profiling of mRNA transcription has become a popular research field. Real-time quantitative RT-PCR (qRT-PCR) has become established as the sensitive method for detecting the expression level of low abundance mRNA, and it usually chooses one or several reference genes to standardize the expression level of target gene. Since the changes in amplification of reference gene can reflect the changes of RNA production, quality or cDNA synthesis efficiency. So choosing an appropriate reference gene can reduce the differences between tested samples. Based on the comparison of Standardization of three frequently-used reference genes (GAPDH, Actin-3, 28srRNA), and decide which is the best way to study gene expression level in silkworm, Bombyx mori.

  Info
Periodical
Advanced Materials Research (Volumes 175-176)
Main Theme
Edited by
Lun Bai and Guo-Qiang Chen
Pages
67-71
DOI
10.4028/www.scientific.net/AMR.175-176.67
Citation
R. Peng, B. J. Su, G. D. Zhao, X. Ji, S. S. Zhao, T. Zhang, R. N. Gao, R. X. Wang, W. D. Shen, Z. G. Wei, "Standardization of Reference Genes in Silkworm, Bombyx Mori", Advanced Materials Research, Vols. 175-176, pp. 67-71, 2011
Online since
January 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ju Mei Wang, Yan Hong Wang, Bin Bin Wang, Ren Hua Fan, Wei De Shen, Bing Li
Chapter 1: Environmental Chemistry and Biology
Abstract:In order to investigate the relationship between the RNA structure and thermal stability, we used real-time PCR method to survey the...
110
Authors: Zhi Guo Miao, Guo Wang Li, Shi Zhu Wang, Xin Yao Chang, Hong Bing Xie, Chang Zhong Liu
Session 2: Materials for Biomedical Engineering Application
Abstract:In this pepar we investigated the developmental patterns of expression of growth hormone (GH) gene in pituitary tissue in pigs of different...
412
Authors: Da Song Lin, Kang Xin He, Qi Xing Zhou, Ye Li, Xiu Jie Xie, Yao Liu
Chapter 2:Materials Science and Bioengineering
Abstract:Chlortetracycline (CTC), antimicrobial chemical, is widely used to treat disease and protect the health of animals. As CTC is poorly absorbed...
230
Authors: Chi Chen, Yan Yan Liu, Zhi Ping Wu, Rui He Niu, Jian Zhong Tan
Chapter 1: Mulberry and Silkworm
Abstract:The silkworm fat body is the organ of many intermediary metabolic processes and sustenance of growth throughout the life, and its proteins...
67