In order to provide scientific proof to anion functional fabrics application research in textile domain, in this article, anion functional fabrics were prepared, the anion generating capacity was determined and the effect of anion fabrics and mice BLA after exercise were first studied. The anion generating capacity shows that: due to the adding of anion function powders to function fabrics, the anion amount released by function fabrics is apparently higher than common fabrics. Washing can not significantly reduce the anion amount of function fabrics releasing. These can indicate that the function fabrics can release anion stable and lasting. The determination of mice’s BLA after swimming showed that Compared to common fabrics, functional fabrics have significant difference (P<0.05). The anions of functional fabrics releasing can reduce animals BLA after exercise and help to eliminate exercise-induced muscle fatigue.