The processing technique of bamboo particle board using laccase-treated calcium lignosulfonate was investigated and optimized. We studied the influence of enzyme amount, calcium ligosulfonate amount and hot pressing temperature on different particle board properties. All three factors were significant difference at different levels. There has significant differences between enzyme amount and Modulus of Rupture (MOR)，Modulus of Elasticity (MOE), Thickness Swelling (TS) (P<0.01)and Internal Bonding Strength (IB) (P<0.1). There has significant differences between Calcium lignosulfonate amount and MOR，TS(P<0.01), IB(P<0.05) and MOE (P<0.1). There has significant differences between hot pressing temperature and MOE，TS(P<0.01) and IB(P<0.1), but no significant differences on MOR. The optimum condition for board making as follows: enzyme amount 50u•g-1，calcium lignosulfonate amount 10%，hot pressing pressure 3.0MPa， 200°C and 7min while 0.75g•cm-3 board density and 10 mm thickness.