This paper demonstrates a method and its implementation on workers’ heart load affected by industry noise, and, to develop a related data processing system. Heart rate variability (HRV) is used as an assessment index. The affected regulation of human’s heart is obtained by large number of noise experiments. This method emphasizes the human heart load among the all factors of labor safeties, the noise influence discipline can be debated scientifically from both engineering physiology and psychology. The results show when to control the industrial noise it is much important to control the ingredient of noise main frequency not the audio-level.