More detailed analysis of chemical compositions, cold plastic deformation and microstructure and properties change of 40Mn and 65Mn steel in recrystallization annealing process were made in the paper. How to get high plasticity and improve the performance of cold pressure processing technology as to cold-rolled 40Mn, 65Mn steel in recrystallization annealing technology, it was suggested that hardness was appropriately sacrificed to ensure the high plasticity. The paper analyzed concretely the reason of the influence of ball Fe3C to plasticity. Recrystallization annealing process and metallographic structure analysis were made. Basically set the process of 40Mn and 65Mn steel recrystallization annealing. Initially carry out the practical application, receive a better effect and have more potential applications foreground.