Anodic aluminium oxide(AAO) templates were fabricated by a two-step anodization method utilizing high purity aluminium sheets. On the basis of getting the optimum preparation parameters of AAO templates, effects of the pretreatment and the first anodization time on morphology of AAO templates were studied. Field emission scanning electron microscope(FESEM) was used to investigate surface morphology of AAO templates. The SEM images indicated that both pretreatment and the first anodization time had a significant influence on morphology of AAO templates. Meanwhile,effect mechanism of pretreatment and the first anodization time on AAO templates morphology was elucidated.