Many natural and synthetic dyes present in industrial effluents are resistant to degradation by conventional treatments. Decolorization of indigo carmine were carried out using a newly isolated ligninolytic fungus strain Ganoderma weberianum TZC1. This strain was shown to decolorize indigo carmine efficiently; the optimal pH and temperature were pH 5.0 and 33°C. A decolorization efficiency of 93% was achieved by cultivation for 1 h at an initial dye concentration of 20 mg/L. Furthermore, G. weberianum was shown to be used efficiently and repeatedly in repeated-batch decolorization operations with the mycelia pellets at the different ages. Our results suggest that G. weberianum has promising applications in dye-containing wastewater decolorization.