Paper Title:
Phylogenetic and Microbial Community Analysis Based on amoA Gene and 16SrDNA in Nitrosification Biofilm Reactor
  Abstract

In order to analyze microbial community and phylogenesis in nitrosification biofilm reactor, a partial stretch of the gene encoding the active-site polypeptide of ammonia monooxygenase (amoA) was amplified and the gene libraries were constructed. The result of gene sequences and phylogenetic analysis showed that Nitrosomonas eutropha was the predominant species in the reactor. Besides, there were also some kinds of ammonia-oxidizing microbe uncultured in the system. PCR-SSCP analysis of 16SrDNA of archaebacteria and eubacterium indicated that with the rising of ammonia oxidation rate, the structure and distribution of microbial community was influenced and the diversity of microbial communities decreased, and the decreasing of specificity in the reactor might be the key factor for the rising of ammonia oxidation rate.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
1051-1056
DOI
10.4028/www.scientific.net/AMR.183-185.1051
Citation
T. Liu, D. Li, J. Zhang, "Phylogenetic and Microbial Community Analysis Based on amoA Gene and 16SrDNA in Nitrosification Biofilm Reactor", Advanced Materials Research, Vols. 183-185, pp. 1051-1056, 2011
Online since
January 2011
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Price
$32.00
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