The urea inclusion was carried out to purify γ-linolenic acid (GLA). The single-factor, double-factor and orthogonal test were employed to optimize technical conditions. Single-factor tests showed that the best ratio of FFA: urea: alcohol was 1:3:8, the inclusion temperature was -15or-20°C and the duration time was 24 hours. Double-factor test indicated the optimal combination was A2B2 or A2B1. The best combination of the orthogonal test was A1B2C2D2 or A4B2C2D2. The ratio of filtrate and urea was 6:2 or 8:3, at the method of isolation was NO. II. Using urea inclusion, GLA purity was raised over 90% with a yield of 80%.