Paper Title:
Plant Regeneration in Sorbus. pohuashanenesis Hedl by Somatic Embryogenesis
  Abstract

Somatic embryogenesis was obtained by using immature zygotic embryos of S. pohuashanesis as explants and emblings were obtained. For induction of somatic embryos, immature zygotic embryos which 30 days old after pollination were cultured on solid MS medium with 1.0 mg•L-1 NAA, 0.1 mg•L-1 6-BA, 500 mg•L-1casein hydrolysate (CH) and 40 g•L-1 sucrose . Inducted somatic embryos were cultured in solid MS medium containing 500 mg•L-1CH and 40 g•L-1 sucrose. After 30 days of culture, many normal cotyledonary embryos were produced. Plantlets were regenerated when somatic embryos were transferred to MS medium with 30 g•L-1 sucrose. The somatic embryos germinated at a germination frequency of approximately 80%, but rate of the plantlets that successfully acclimated and continued growing was 40% in the greenhouse.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
1462-1466
DOI
10.4028/www.scientific.net/AMR.183-185.1462
Citation
L. Yang, Y. H. Li, H. L. Shen, "Plant Regeneration in Sorbus. pohuashanenesis Hedl by Somatic Embryogenesis", Advanced Materials Research, Vols. 183-185, pp. 1462-1466, 2011
Online since
January 2011
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$32.00
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