Paper Title:
Research on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast
  Abstract

The factors of species of the medium, spawn age, species of the enzyme, time of hydrolysis, hydrolysis temperature, osmotic pressure stabilizer and regeneration medium, which have important effects on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast, were discussed in this paper. These results showed that the hydrolysis condition can be described as following: Chaetomium cupreum mycelium with spawn age about 48h, the concentration of the lytic enzymes about 2% and the time of enzymatic hydrolysis 2h. The regeneration condition of the protoplast is that: Chaetomium cupreum mycelium with the spawn age 6h, the concentration of the lytic enzymes 2%, the temperature of 25°C and the time of enzymatic hydrolysis 1.5h. We can get the Chaetomium cupreum protoplast, regenerating in the RA Medium with the sorbitol, which regeneration can be over 50%.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
1522-1526
DOI
10.4028/www.scientific.net/AMR.183-185.1522
Citation
H. M. Yue, Q. Yang, "Research on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast", Advanced Materials Research, Vols. 183-185, pp. 1522-1526, 2011
Online since
January 2011
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Price
$32.00
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