Tilia amurensis is one of the most valuable broad-leaved trees in northeast China. It is very important to study cutting because T. amurensis seeds have dormancy characteristics, which caused a great obstacle for its propagation. In this study, the effect of different factors and levels on rooting rate of T. amurensis cuttings were investigated by a orthogonal design (L1645). The age of cutting, hormone concentration and matrix all affected rooting rate, among which matrix was the main factor, followed by ages of the mother tree and the hormone concentration. From three to four years old cuttings treated with a hormone concentration of 1.0 × 10-4 and cultured in vermiculite and sand could get higher rooting rate. The best combination of treatments was to use 3-4a aged cuttings treated with IAA(1.0×10-4) and dig into the sand for 16h, which rooting rate got to 63.3%.