The feasibility of producing biodiesel from Xanthoceras sorbiflia Bunge seed oil was studied. Biodiesel was prepared from refined Xanthoceras sorbiflia Bunge seed oil using methanol and sodium hydroxide in an alkali-catalyzed transesterification process. The refining process consisting of alkali deacidifying was developed to produce biodiesel from crude Xanthoceras sorbiflia Bunge seed oil. The acid value of oil was reduced from the initial 1.28mg- KOH/g-oil to below 1.0 mg-KOH/g-oil in 1 h under the conditions of NaOH 18% , excess alkali quantity 0.2wt% of oil at 30°C. The yield of biodiesel developed by refined Xanthoceras sorbiflia Bunge seed oil was higher than 89% in 60 min using NaOH catalyst that amount approximately1wt.% compared to Xanthoceras sorbiflia Bunge seeds oil as catalyst and a molar ratio of methanol to oil 6:1 at 60°C.