Due to influence of size effect, the force properties in nanoscale are greatly different from those in macroscale and the traditional models of operation are becoming difficult to meet the development of nanoscale manipulation. To provide guiding theory for practical nano-manipulation, the nanoscale forces of contact and non-contact operation of nanowires are analyzed for nano-manipulation in SEM. The Vander Waals models among the probe, nanowire and substrate are modeled according to the force properties in nanoscale, and then the simplified models are simulated with MATLAB. The influence degree of various factors and the relationship of them during the operation are obtained. At last, experimental system is established to verify the correctness of the proposed models.