The dyeability of PLA fabric with the natural emodin dye has been investigated. The effect of different factors, which are dyeing temperature, dyeing time, dye bath pH value, bath ratio, dye concentration and mordants, has been studied. The color of each dyed sample was evaluated in terms of the K/S value, and the color fastness to soaping and crocking were tested according to the ISO standards. The results showed that the dyed fabrics have a bright color and an excellent color fastness. As a result, emodin dyes PLA is a good approach to develop “green clothes” and has broad prospect.