Paper Title:
Spectroscopic Studies on the Interaction between 2-Chlorophenol and Catalase
  Abstract

The interaction characteristics of 2-chlorophenol (2-CP) with catalase (CAT) were investigated by employing fluorescence spectroscopy and UV-Vis absorption spectroscopy. The intrinsic fluorescence of CAT was quenched distinctly by 2-CP. The quenching mechanism of fluorescence of CAT by 2-CP was observed to be a static quenching procedure. The thermodynamic parameters indicated that the binding reaction was spontaneous and the hydrophobic force played the major role in stabilizing the 2-CP-CAT complex. The binding constant was 1.18×10-4 L/mol. The binding distance r and critical distance R0 was 1.90 nm and 1.64 nm, respectively.

  Info
Periodical
Advanced Materials Research (Volumes 183-185)
Chapter
Environmental Biotechnology
Edited by
Yanguo Shi and Jinlong Zuo
Pages
206-210
DOI
10.4028/www.scientific.net/AMR.183-185.206
Citation
Q. Y. Liu, "Spectroscopic Studies on the Interaction between 2-Chlorophenol and Catalase", Advanced Materials Research, Vols. 183-185, pp. 206-210, 2011
Online since
January 2011
Authors
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Price
$32.00
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